Nowadays, due to environmental concerns and the reduction in fossil fuel resources, biodiesel is being considered as a renewable alternative or supplement to fossil diesel. In this research, the transesterification method was used to produce biodiesel from low-cost and non-edible linseed oil in the presence of commercial calcium oxide as the catalyst. In addition, the time-consuming and costly calcination step was omitted and a deep eutectic co-SOLVENT of choline chloride/glycerol (2:1) was used to activate the catalyst. The catalyst characterization was studied using X-ray diffraction(XRD) and Fourier transform infrared (FTIR). To ensure the formation of fatty acid methyl esters (FAME), the purity of FAME in the produced biodiesel was calculated using gas chromatography method. Then, the effect of two reaction parameters of the catalyst and the DES concentration on the FAME purity was investigated at a constant temperature of 65 °C, stirring speed of 500 rpm, reaction time of 180 minutes and the methanol to oil molar ratio of 9.5:1. Finally, the optimal catalyst and the DES concentration were obtained as 10% and 14%, respectively. The effect of temperature and time on the FAME purity was investigated and a kinetic study of the reaction suggested a pseudo-first-order reaction with a rate constant of 0.0311 at 65 °C and activation energy of 22.45 kJ/mol.